Application of liquid chromatography-electrospray ionization mass spectrometry for study of steroid-converting enzymes.
نویسندگان
چکیده
A high-performance liquid chromatography-atmospheric pressure ionization-electrospray ionization mass spectrometry (HPLC-API-ESI-MS) method was developed for the analysis of steroids in a study of steroid-converting enzymes. Separations ware done on a Zorbax Eclipse XDB-C18 column (eluted with a linear methanol-water-acetic acid gradient) and identification of the steroids involved was done by API-ESI-MS using positive ion mode and extracted ion analysis. The applicability of the present method for studying steroid metabolism was proven in assaying two steroid-converting enzymes (20beta-hydroxysteroid dehydrogenase and 11beta-hydroxysteroid dehydrogenase) in various biological samples (rat and chicken intestine, chicken oviduct).
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ورودعنوان ژورنال:
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
دوره 800 1-2 شماره
صفحات -
تاریخ انتشار 2004